Product Information

Do you need oligonucleotides with degeneracies or advanced purification?

No problem—T4 Oligo can synthesize your primers at scales of 25 nM, 50 nM, 100 nM, 200 nM, and 1000 nM, including degeneracies and specialized purification methods. If you need more information about the purification type that best suits your needs, or if you’d like to be assigned a T4 Oligo specialist, please contact us at: ventas@t4oligo.com

Technical Information

Resuspension and storage of your oligos.

To resuspend or reconstitute your oligos and probes, add the volume of water or buffer indicated on the synthesis certificate and follow these steps:

• Centrifuge the tube to ensure the pellet is at the bottom.
• Add the appropriate volume indicated on the synthesis certificate.
• Wait a few minutes to allow the pellet to hydrate, making resuspension easier.
• Place the tube on a vortex mixer for 15 seconds.
• It is recommended to resuspend Cy®-labeled probes in TE buffer at pH 7.

Scale vs. Yield

Scale
The synthesis scale refers to the raw materials used—in this case, the support and standardized protocol applied in oligonucleotide synthesis.

Yield
Yield corresponds to the amount of final product obtained. The yield of an oligonucleotide depends on its length, sequence, modifications, and purification method.

Purification vs. Yield

Yield refers to the amount of final product obtained. The yield of an oligonucleotide depends on its length, sequence, modifications, and purification method.
In summary, the relationship between purification and purity can be described as follows:

Lower purification = higher yield.

- Higher purification = lower yield.

HPLC vs. PAGE = higher yield with slightly lower purity.

PAGE vs. HPLC = slightly higher purity but lower yield.

Cartridge vs. HPLC or PAGE = equal or higher purity and yield, but limited to certain oligos.

 

Contrary to common belief, the purity of an oligo does not primarily refer to the presence of non-nucleic acid compounds in the final product, but rather to the amount of “usable” or complete oligo delivered to the customer. During synthesis, a small proportion of truncated or branched oligos is generated—this is normal within a certain range. However, these truncated sequences must be minimal or nearly absent by the time the oligo reaches your lab. According to T4 Oligo’s quality standards, if 99.1% of the oligos are not complete in sequence, the product is discarded for being considered low quality. Our standard is ten times stricter than that of our closest competitor, based on comparative studies conducted in our laboratory.

Producing complete oligos depends both on efficient synthesis—which in turn requires high-quality equipment, reagents, and synthesis expertise—and on the purification process that follows the oligo’s release from the synthesizer.

In the case of fluorescently labeled probes or oligos, purification is essential not only to ensure a majority population of complete oligos that will efficiently recognize their target strand, but also to remove any fluorophore that failed to bind to the DNA during synthesis. Free fluorophores still emit fluorescence, generating background noise.

Performance and Quality

How do we ensure our quality?

Whatever the application for which you require oligonucleotides, T4 Oligo offers the quality and technological capability your experiments demand—with unmatched delivery times in Mexico and no extra costs.

With T4 Oligo, you can rest assured that your oligonucleotides will arrive at your lab within just a few days, backed by a certificate of analysis and quality assurance that guarantees your reagents have been carefully synthesized under our policy and certification in Good Manufacturing Practices (GMP). To learn more about our certifications and quality assurance processes, please visit the following link: